CC-3875 RbcS2:5.7 kbp
$30.00
From Arminio Boschetti, University of Bern, August 2001
CC-3875 through CC-3893 are a collection of transformants of the T60-3 Rubisco knockout mutant created by Khrebtukova and Spreitzer (CC-4415).
CC-3875 was created by transformation of T60-3 (rbcS-T60-3T60-3) with a modified plasmid pRbcS-2. The original plasmid, a pUC19-vector containing the genomic BamHI/EcoRI fragment encoding rbcS-2 [EMBL/GenBank accession X04471], total length 10.5 kb, was shortened by elimination of an unknown DNA sequence of about 5 kb. Transformation resulted in complementation of T60-3 to normal photoautotrophy. CC-3875 was used as a control strain for CC-3876 through CC-3889, which have mutations in the RBCS transit peptide.
Khrebtukova I, Spreitzer RJ (1996) Elimination of the Chlamydomonas gene family that encodes the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Proc Natl Acad Sci USA 93:13689-13693