* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-3927 pf6-2 arg7 rescue mt- ET
$30.00
From Mary Porter, University of Minnesota, October 2001
This is a strain with wild type motility obtained by cotransformation of pf6-2 arg7 with pARG7.8 and an epitope-tagged construct of the PF6 gene, pSET-41. The PF6 gene product can be detected with antibiodies against the HA epitope tag, as described by Rupp et al. 2001.
Rupp G, O'Toole E, Porter ME (2001) The Chlamydomonas PF6 locus encodes a large alanine/proline-rich polypeptide that is required for assembly of a central pair projection and regulates flagellar motility. Mol Biol Cell 12:739-751