CC-4044 CR04 (amt4) mt+
$30.00
From William Inwood and Sydney Kustu, UC Berkeley, January 2005
Phenotype: inhibitor resistant (methylammonium)
This is a spontaneous methylammonium-resistant mutant isolated in the 4A+ background (CC-4051). CR04 contains a single base pair substitution, which changes a GGC codon (glycine) to GAC (aspartate) in exon 5 of the AMT4 gene. The change occurs at amino acid residue 236 within putative transmembrane segment 5. Based on the level of resistance to methylammonium and methylammonium uptake characteristics, this non-conservative change in a critical region appears to result in an amt4 null allele.
Kim KS, Feild E, King N, Yaoi T, Kustu S, Inwood W (2005) Spontaneous mutations in the ammonium transport gene AMT4 of Chlamydomonas reinhardtii. Genetics 170:631-644