* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-4046 CR46 (amt4) mt+
$30.00
From William Inwood and Sydney Kustu, UC Berkeley, January 2005
Phenotype: inhibitor resistant (methylammonium)
This is a spontaneous methylammonium-resistant mutant isolated in the 4A+ background (CC-4051). CR46 has an insertion of the Tcr1 transposon immediately upstream of the AMT4 translational start, leading to a duplication of the target sequence GTCGCAGG at -15 to -8. The insert is within the 5′ untranslated leader of the AMT4 transcript and CR46 retains residual Amt4 function.
Kim KS, Feild E, King N, Yaoi T, Kustu S, Inwood W (2005) Spontaneous mutations in the ammonium transport gene AMT4 of Chlamydomonas reinhardtii. Genetics 170:631-644