CC-4052 CR02 (amt4) mt+
$30.00
From William Inwood and Sydney Kustu, January 2005
Phenotype: inhibitor resistant (methylammonium)
This is a spontaneous methylammonium-resistant mutant isolated in the 4A+ background (CC-4051). CR02 carries a 2-bp deletion near the end of exon 1 of the AMT4 gene, encoding an ammonium transport protein. The mutation leads to a frame shift after correct translation of the first 68 amino acids, ending within predicted transmembrane segment 1. These are followed by 53 incorrect amino acids and a stop. The normal protein is 499 amino acids in length. This lesion in AMT4 appears to be a null allele.
Kim KS, Feild E, King N, Yaoi T, Kustu S, Inwood W (2005) Spontaneous mutations in the ammonium transport gene AMT4 of Chlamydomonas reinhardtii. Genetics 170:631-644