CC-4164 gam10 mt-
$30.00
From Charlene Forest, Brooklyn College, August 2007
Phenotype: does not mate when grown at high temperature
This is a temperature-sensitive, sex-limited mutant defective in gamete fusion. When incubated overnight in N-free medium at the restrictive temperature, gam10 mt- cells can agglutinate with mt+ gametes, signal, activate their mating structures and adhere via their mating structures with activated wt+ mating structures but they cannot fuse. The gam11 mutant has a similar phenotype, but was independently isolated.
Forest CL (1983) Specific contact between mating structure membranes observed in conditional fusion-defective Chlamydomonas mutants. Exp Cell Res 148:143-154
Forest CL (1987) Genetic control of plasma membrane adhesion and fusion in Chlamydomonas gametes. J Cell Sci 88:613-621
Forest CL, Ojakian GK (1989) Mating Structure Differences Demonstrated by Freeze-Fracture Analysis of Fusion-Defective Chlamydomonas Gametes. The Journal of Protozoology 36:548-556