CC-4192 lts1-204::Rh1-GFP [Kustu CR-467]
$30.00
From Corinne Yoshihara, Sydney Kustu lab, University of California, Berkeley, April 2008
Phenotype: carotenoid deficient
This strain was derived by transformation of lts1-204 with an Rh1-GFP fusion construct (TM1-4) containing Rh1 transmembrane regions 1-4. The lts1 mutant was used as a recipient in order to eliminate chlorophyll fluorescence, which interfered with detection of GFP. Please see CC-4109 for more information on the LTS1 [PSY1] locus.
McCarthy SS, Kobayashi MC, Niyogi KK (2004) White mutants of Chlamydomonas reinhardtii are defective in phytoene synthase. Genetics 168:1249-1257
Yoshihara C, Inoue K, Schichnes D, Ruzin S, Inwood W, Kustu S (2008) An Rh1-GFP fusion protein is in the cytoplasmic membrane of a white mutant strain of Chlamydomonas reinhardtii. Mol Plant 1:1007-1020