CC-3388 psbA (A251I*) mt+
$30.00
From Anita Lardans, Boynton-Gillham laboratory, Duke University
This strain contains a site-directed mutation A251I in the psbA gene, and a silent mutation (indicated by * in the strain name) creating an SspI site by substituting a T for a C at position 247. CC-125 wild-type cells were co-transformed with the psbA plasmid and with plasmid P-228, which confers spectinomycin resistance by a mutation in the gene encoding the chloroplast 16S ribosomal RNA.
The A251I mutation confers resistance to atrazine, bromacil and metribuzin, but not to DCMU, and results in poor photoautotrophic growth, although it is not as severely impaired as the Leu substitution at the same residue (see CC-3389).
Förster B, Heifetz PB, Lardans A, Boynton JE, Gillham NW (1997) Herbicide resistance and growth of D1 Ala251mutants in Chlamydomonas. Z Naturforsch 52:654-664
Lardans A, Gillham NW, Boynton JE (1997) Site-directed mutations at residue 251 of the photosystem II D1 protein of Chlamydomonas that result in a nonphotosynthetic phenotype and impair D1 synthesis and accumulation. J Biol Chem 272:210-216