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CC-4284 bop5-2 (6F5) mt+

Name: CC-4284 bop5-2 (6F5) mt+
SKU: CC-4284
Availability: InStock

$30.00

From Mary Porter, U of MN, March 2009

The 6F5 mutant was isolated by Steve Myster in the Porter lab using insertional mutagenesis of A54 e18 (CC-2929). The pMN56 plasmid containing the NIT1 gene integrated into the BOP5 locus encoding the IC138 intermediate chain of the I1 inner arm dynein. The insertion event was associated with the deletion of ~20 kb of genomic gene, including most of the IC138 gene. IC138 fails to assemble into the axoneme along with at least three other I1 dynein subunits (IC97, LC7b, FAP120) known as the IC138 sub-complex. There is also a second uncharacterized motility mutation in the strain.

The bop (bypass of paralysis) mutants were isolated by Dutcher et al. in a pf10 background, by selection for restoration of motility.

Dutcher SK, Gibbons W, Inwood WB (1988) A genetic analysis of suppressors of the PF10 mutation in Chlamydomonas reinhardtii. Genetics 120:965-976

Dutcher SK, Power J, Galloway RE, Porter ME (1991) Reappraisal of the genetic map of Chlamydomonas reinhardtii. J Hered 82:295-301

Dutcher SK, Gibbons W, Inwood WB (1988) A genetic analysis of suppressors of the PF10 mutation in Chlamydomonas reinhardtii. Genetics 120:965-976

Dutcher SK, Power J, Galloway RE, Porter ME (1991) Reappraisal of the genetic map of Chlamydomonas reinhardtii. J Hered 82:295-301

CC-4284 bop5-2 (6F5) mt+

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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