CC-4368 css1 spr-u-1-6-2 er-u-11 mt+ [Herrin 7151]

$30.00

From David L. Herrin, University of Texas at Austin, September 2010, his 7151

The css1 mutation suppresses defects in splicing of the chloroplast 23S (Cr.LSU) intron and also the psbA fourth intron. It was obtained as follows: Mets strain 2137 (CC-1021, also CC-3269) was transformed with a construct that contained the spr-u-1-6-2 mutation from plasmid P-183 (a single base pair change in the 16S rRNA) and the chloroplast 23S (Cr.LSU) intron, in which site-directed changes had been made. Transformants were selected by spectinomycin resistance. A transformant defective in Cr.LSU self-splicing (P4125A), which grew slowly and was light-sensitive, was then used to isolate suppressor mutations that restored Cr.LSU splicing.

Li F, Holloway SP, Lee J, Herrin DL (2002) Nuclear genes that promote splicing of group I introns in the chloroplast 23S rRNA and psbA genes in Chlamydomonas reinhardtii. Plant J 32:467-480

Liu Y, Lee D, Ialicicco M, Akkinepalli H, Morreale G, Leung H, Scippa GS, Greenland A, Mackay I (2012) Genotyping SSR length variants by isothermal DNA amplification. Genome 55:691-695