CC-4381 ptx6-2/bbs7-1:: TOC1 agg1 mt+
$30.00
From Deborah Cochran, George Witman lab, University of Massachusetts Medical School, November 2010, their T87
This mutant was obtained by transformation of strain g1 (nit1, agg1, mt+) with the NIT1 plasmid pMN24. It is phototaxis negative, photoshock positive. Lechtreck et al. showed that it is an allele at the BBS7 locus.
Witman included the following note: “The ptx6-1 (T197) and ptx6-2 (T87) strains in the Witman collection now (2010) appear to be identical. Hence, Lechtreck et al. (2010) equated bbs7-1 with ptx6-1, although the strain used was T87 (ptx6-2). The mutation in the BBS7 gene of T87 involves a retrotransposon TOC1 footprint whereas Pazour et al. (1995) reported that the ptx6-1 strain contained pUC119 sequence that segregated with the nonphototactic phenotype but did not encode a functional nitrate reductase gene. It is not known if the bbs7-1 strain (i.e., T87) carries a second, insertional, mutation linked to the BBS4 gene, or if the original ptx6-1 and ptx6-2 alleles were different but ptx6-1 has been lost. In any case, this strain should be referred to simply as bbs7-1. If relevant, anyone working with this strain in the future should check to see if it still contains pUC119 sequence that might be disrupting a second gene.”
Pazour GJ, Sineshchekov OA, Witman GB (1995) Mutational analysis of the phototransduction pathway of Chlamydomonas reinhardtii. J Cell Biol 131:427-440
Lechtreck KF, Johnson EC, Sakai T, Cochran D, Ballif BA, Rush J, Pazour GJ, Ikebe M, Witman GB (2009) The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagella. J Cell Biol 187:1117-1132