* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-4407 sup-pf-3::PF2-GFP mt- [5G]

Name: CC-4407 sup-pf-3::PF2-GFP mt- [5G]
SKU: CC-4407
Availability: InStock

$30.00

From Mary Porter, University of Minnesota, April 27, 2011

This rescued strain was created by transformation of sup-pf-3 mt- (CC-1399) with PF2-GFP and pSI103 with selection on paromomycin. Screened for rescue of motility and presence of GFP signal in the flagella. Strong PF2-GFP signal in flagella, weak expression of sup-pf-3 gene products (truncated PF2) by Western blot.

Huang B, Ramanis Z, Luck DJ (1982) Suppressor mutations in Chlamydomonas reveal a regulatory mechanism for Flagellar function. Cell 28:115-24

Bower R, Tritschler D, Vanderwaal K, Perrone CA, Mueller J, Fox L, Sale WS, Porter ME (2013) The N-DRC forms a conserved biochemical complex that maintains outer doublet alignment and limits microtubule sliding in motile axonemes. Mol Biol Cell 24:1134-52

Locus:
SUP-PF3
Chromosome:
11

CC-4407 sup-pf-3::PF2-GFP mt- [5G]

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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