* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-5133 hydA2-1 mt+
$30.00
From Sarah D’Adamo, Matthew Posewitz lab, Colorado School of Mines, Golden CO, July 2015
Mutation in HYDA2 by insertion of pSL72 plasmid, carrying AphVIII gene (paromomycin resistance). Crossed 4X with D66 (CC-4425 cw15 nit2-203 mt+) and CC-124.
HYDA2: chromosome_9:1740957..1745328
Meuser JE, D'Adamo S, Jinkerson RE, Mus F, Yang W, Ghirardi ML, Seibert M, Grossman AR, Posewitz MC (2012) Genetic disruption of both Chlamydomonas reinhardtii [FeFe]-hydrogenases: Insight into the role of HYDA2 in H₂ production. Biochem Biophys Res Commun. 417:704-9