* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-5134 trk2-1 act1 hkr1 mt- [10KDP1]
$30.00
From David Polley, Wabash College, July 2015
Phenotype: requires high potassium concentration
This strain requires high potassium (concentrations greater than 1mM) for growth. Wild-type growth rates require 10mM K. Use agarose solidified medium when testing for phenotype on plate cultures.
This mutant was UV induced in a trk2-1 act1 recombinant produced from a cross between CC-1715 (pf18 nic2 act1 mt-) and CC-3832. Phenotype expression requires trk2-1 background, but phenotype expression in trk3 or trk1 background has not been tested.
Polley LD (1999) Genetic analysis of mutant clones of Chlamydomonas reinhardtii defective in potassium transport. Mol. Gen. Genet. 261, 275-280