* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-5159 ift74-1 mt+
$30.00
From Jason Brown, George Witman lab, University of Massachusetts Medical School, October 2015
The ift74-1 allele was identified in strain BB12 generated by insertional mutagenesis of strain D10 (CC-124 background) with the 1.7 kb HindIII fragment of pHyg3 (Berthold et al., 2002, Protist). Strain ift74-1 is a product of two backcrosses of BB12 to CC-125. A more complete description of the parental strain is in Brown et al., 2015.
ift74-1 expresses a truncated IFT74 protein lacking 196 aa from its N terminus. It assembles short flagella when maintained in M medium without aeration.
Brown JM, Cochran DA, Craige B, Kubo T, Witman GB (2015) Assembly of IFT trains at the ciliary base depends on IFT74. Curr Biol. 25: 1583-1593