* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-5160 ift74-1::IFT74 rescue mt+
$30.00
From Jason Brown, George Witman lab, University of Massachusetts Medical School, October 2015
This strain was generated by transformation of ift74-1 with the plasmid pIFT74aphVIII containing wild-type IFT74 genomic sequence and the paromomycin-resistance cassette from pKS-aphVIII-lox (Heitzer and Zschoernig, 2007, BioTechniques).
This strain has restored wild-type flagella assembly.
Brown JM, Cochran DA, Craige B, Kubo T, Witman GB (2015) Assembly of IFT trains at the ciliary base depends on IFT74. Curr Biol. 25: 1583-1593