* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-5161 ift74-2 mt-
$30.00
From Jason Brown, George Witman lab, University of Massachusetts Medical School, October 2015
The ift74-2 allele was identified in strain 11C#3 generated by insertional mutagenesis of strain oda2 (Kamiya, 1988, J. Cell. Biol.) with the 1.7 kb HindIII fragment of pHyg3 (Berthold et al., 2002, Protist). The ift74-2 strain is the result of a backcross to CC-124 (137c mt-) that replaced the oda2 allele with a wild-type ODA2 allele.
This strain almost completely fails to assemble flagella.
Brown JM, Cochran DA, Craige B, Kubo T, Witman GB (2015) Assembly of IFT trains at the ciliary base depends on IFT74. Curr Biol. 25: 1583-1593