CC-556 ac208 mt-
$30.00
From Ursula Goodenough, Harvard University, May 1978
Phenotype: requires acetate on copper-containing medium.
The ac208 mutation was UV-induced in a germinating zygote in Levine’s laboratory.
This mutant is acetate-requiring on copper-containing medium. Copper deprivation induces expression of cytochrome c6, which bypasses the requirement for acetate. This mutant has been extensively studied by Merchant and colleagues. Quinn et al. showed that the alteration is a frameshift mutation in the gene encoding plastocyanin.
We have two stocks of this mutant in mating type minus, this one and CC-1729 in recent years we have generally sent out CC-1729, although both are listed in the core collection.
Hill KL, Merchant S (1992) In Vivo Competition between Plastocyanin and a Copper-Dependent Regulator of the Chlamydomonas reinhardtii Cytochrome c(6) Gene. Plant Physiol 100:319-26
Quinn J, Li HH, Singer J, Morimoto B, Mets L, Kindle K, Merchant S (1993) The plastocyanin-deficient phenotype of Chlamydomonas reinhardtii Ac-208 results from a frame-shift mutation in the nuclear gene encoding preapoplastocyanin. J Biol Chem 15:7832-41