CC-4536 cw806 mt- [Smyth 806]

$30.00

From Peter Hegemann, Humboldt University, Berlin, July 2012

Phenotype: altered phototaxis

This is the original strain that Bob Smyth isolated in the Ebersold lab. The isolate was derived as a spontaneous cell-wall-less mutant from a single colony of Smyth strain 806. It shows negative phototaxis at all photon irradiances and has some cell-wall defect.

This strain has a weaker cell wall but it is not cell wall deficient. It is relatively stable but has the advantage that it can be lysed in a so-called Yeda Press (also named Parr Bomb) which is much milder that a French Press or Fluidizer. Thus internal membranes can be isolated with higher purity.

Hegemann P, Hegemann U, Foster KW (1988) Reversible bleaching of Chlamydomonas reinhardtii rhodopsin in vivo. Photochem Photobiol 48:123-8