* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-4620 76-5EN mt+ (rbc-3-76-5EN) original isolate
$30.00
From Robert J. Spreitzer, University of Nebraska, March 2014
Phenotype: requires acetate, sensitive to light
This nuclear mutant was isolated by Hong and Spreitzer (1994) by screening a collection of acetate-requiring mutants for lack of complementation with a known Rubisco rbcL mutant. The mutant is specifically defective in chloroplast rbcL mRNA accumulation. This strain has been maintained with acetate medium in darkness since its isolation.
Hong S, Spreitzer RJ (1994) Nuclear mutation inhibits expression of the chloroplast gene that encodes the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Plant Physiol 106:673-678