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CC-4621 76-5EN mt+ (rbc-3-76-5EN) non-pf2 progeny used for genetic analysis

Name: CC-4621 76-5EN mt+ (rbc-3-76-5EN) non-pf2 progeny used for genetic analysis
SKU: CC-4621
Availability: InStock

$30.00

From Robert J. Spreitzer, University of Nebraska, March 2014

Phenotype: requires acetate, sensitive to light

The 76-5EN nuclear mutant was isolated by Hong and Spreitzer (1994) by screening a collection of acetate-requiring mutants for lack of complementation with a known Rubisco rbcL mutant. The mutant was crossed with pf2 mt- to confirm mendelian inheritance, and this mt+ progeny clone was retained for analysis. The mutant is specifically defective in chloroplast rbcL mRNA accumulation. This strain has been maintained with acetate medium in darkness since its isolation.

Hong S, Spreitzer RJ (1994) Nuclear mutation inhibits expression of the chloroplast gene that encodes the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Plant Physiol 106:673-678

CC-4621 76-5EN mt+ (rbc-3-76-5EN) non-pf2 progeny used for genetic analysis

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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