CC-4624 R107-4A pf2 mt+ (revertant of 76-5EN)
$30.00
From Robert J. Spreitzer, University of Nebraska, March 2014
This strain was recovered from a cross between R107-4A mt+ and 76-5EN pf2 mt- (Hong 1996). It has been maintained with acetate medium in darkness since its isolation. The R107-4A photosynthesis-competent revertant was selected from the Rubisco rbcL-mRNA deficient 76-5EN nuclear mutant (Hong and Spreitzer 1994, 1998). When R107-4A mt+ was crossed with “wild-type” mt-, only wild-type progeny were obtained (Hong and Spreitzer 1998). When R107-4A mt+ was crossed with 76-5EN pf2 mt-, the revertant (wild-type) phenotype segregated in a 2:2 pattern with the 76-5EN acetate-requiring phenotype, which indicates that the revertant results from a nuclear-gene mutation (Hong 1996). R107-4A likely results from true reversion or intragenic suppression within the 76-5EN gene.
Hong S (1996) Nuclear mutations affect the catalysis, stability, and expression of chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Ph. D. thesis, University of Nebraska
Hong S, Spreitzer RJ (1994) Nuclear mutation inhibits expression of the chloroplast gene that encodes the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Plant Physiol 106:673-678
Hong S, Spreitzer RJ (1998) Nuclear-gene mutations suppress a defect in the expression of the chloroplast-encoded large subunit of ribulosebisphosphate carboxylase/oxygenase. Plant Physiol 116:1387-1392