CC-4626 S107-4B pf2 mt+ (suppressor of 76-5EN)
$30.00
From Robert J. Spreitzer, University of Nebraska, March 2014
Phenotype: requires acetate at 35 °C, temperature-conditional
Revertant R107-4B mt+ (76-5EN/S107-4B) was crossed with pf2 mt- to recover the nuclear intergenic suppressor S107-4B in the absence of 76-5EN. S107-4B alone does not affect the level of Rubisco rbcL mRNA, but it has a photosynthesis-deficient temperature-conditional phenotype (Hong and Spreitzer 1998). It can grow on minimal medium at 25 °C, but dies on minimal medium at 35 °C. This strain has been maintained with acetate medium in darkness since its isolation.
Hong S, Spreitzer RJ (1994) Nuclear mutation inhibits expression of the chloroplast gene that encodes the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Plant Physiol 106:673-678
Hong S, Spreitzer RJ (1998) Nuclear-gene mutations suppress a defect in the expression of the chloroplast-encoded large subunit of ribulosebisphosphate carboxylase/oxygenase. Plant Physiol 116:1387-1392