* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-4628 R107-4D mt+ (revertant of 76-5EN)

Name: CC-4628 R107-4D mt+ (revertant of 76-5EN)
SKU: CC-4628
Availability: InStock

$30.00

From Robert J. Spreitzer, University of Nebraska, March 2014

Phenotype: requires acetate at 35 °C, temperature-conditional

This photosynthesis-competent revertant was selected from the Rubisco rbcL-mRNA deficient 76-5EN nuclear mutant (Hong and Spreitzer 1994, 1998). It can grow on minimal medium at 25 °C, but dies on minimal medium at 35 °C because of decreased expression of Rubisco. R107-4D is a double mutant (76-5EN/S107-4D) that results from a nuclear-gene intergenic suppressor (S107-4D) of 76-5EN. When separated from 76-5EN, S107-4D does not have a temperature-conditional phenotype (Hong and Spreitzer 1998), and does not appear to affect Rubisco expression. This strain has been maintained with acetate medium in darkness since its isolation.

Hong S, Spreitzer RJ (1994) Nuclear mutation inhibits expression of the chloroplast gene that encodes the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Plant Physiol 106:673-678

Hong S, Spreitzer RJ (1998) Nuclear-gene mutations suppress a defect in the expression of the chloroplast-encoded large subunit of ribulosebisphosphate carboxylase/oxygenase. Plant Physiol 116:1387-1392

CC-4628 R107-4D mt+ (revertant of 76-5EN)

Products

Support

Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

Cart