CC-4629 S107-4D mt- (suppressor of 76-5EN)
$30.00
From Robert J. Spreitzer, University of Nebraska, March 2014
This strain was recovered from a cross between R107-4D mt+ and “wild-type” mt- (Hong and Spreitzer 1998). It has been maintained with acetate medium in darkness since its isolation. The R107-4D photosynthesis-competent revertant was selected from the Rubisco rbcL-mRNA deficient 76-5EN nuclear mutant (Hong and Spreitzer 1994, 1998). When R107-4D mt+ was crossed with “wild-type” mt-, tetrad analysis indicated that R107-4D resulted from a nuclear-gene intergenic-suppressor mutation (S107-4D). Thus, S107-4D suppresses 76-5EN by restoring Rubisco holoenzyme expression.
Hong S, Spreitzer RJ (1994) Nuclear mutation inhibits expression of the chloroplast gene that encodes the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Plant Physiol 106:673-678
Hong S, Spreitzer RJ (1998) Nuclear-gene mutations suppress a defect in the expression of the chloroplast-encoded large subunit of ribulosebisphosphate carboxylase/oxygenase. Plant Physiol 116:1387-1392