CC-4633 S118-25G pf2 mt+ (suppressor of 76-5EN)
$30.00
From Robert J. Spreitzer, University of Nebraska, March 2014
This strain was recovered from a cross between R118-25G mt+ and pf2 mt- (Hong and Spreitzer 1998). It has been maintained with acetate medium in darkness since its isolation. The R118-25G photosynthesis-competent revertant was selected from the Rubisco rbcL-mRNA deficient 76-5EN nuclear mutant (Hong and Spreitzer 1994, 1998). When R118-25G mt+ was crossed with “wild-type” pf2 mt-, tetrad analysis indicated that R118-25G resulted from a nuclear-gene intergenic-suppressor mutation (S118-25G). Thus, S118-25G suppresses 76-5EN by restoring Rubisco holoenzyme expression.
Hong S, Spreitzer RJ (1994) Nuclear mutation inhibits expression of the chloroplast gene that encodes the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Plant Physiol 106:673-678
Hong S, Spreitzer RJ (1998) Nuclear-gene mutations suppress a defect in the expression of the chloroplast-encoded large subunit of ribulosebisphosphate carboxylase/oxygenase. Plant Physiol 116:1387-1392