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CC-4643 68-11AR mt- (rbc-2-68-11AR) non-pf2 progeny used for genetic analysis

Name: CC-4643 68-11AR mt- (rbc-2-68-11AR) non-pf2 progeny used for genetic analysis
SKU: CC-4643
Availability: InStock

$30.00

From Robert J. Spreitzer, University of Nebraska, March 2014

Phenotype: requires acetate at 35 °C, temperature-conditional

The 68-11AR nuclear mutant was isolated by screening for temperature-conditional, acetate-requiring mutants (Spreitzer et al. 1988). The mutant was crossed with pf2 mt- to confirm mendelian inheritance, and this mt- progeny clone was retained for further genetic analysis. 68-11AR grows on minimal medium at 25 °C, but dies on minimal medium at 35 °C. The nuclear mutation is not in the Rubisco rbcS genes, but it causes a decrease in Rubisco stability and CO2/O2 specificity (Gotor et al. 1994). Some clones of 68-11AR have been observed to become acetate-requiring at 25 °C after many years of culture. This strain has been maintained with acetate medium in darkness since its isolation.

Spreitzer RJ, Al-Abed SR, Huether MJ (1988) Temperature-sensitive, photosynthesis-deficient mutants of Chlamydomonas reinhardtii. Plant Physiol 86:773-777

Gotor C, Hong S, Spreitzer RJ (1994) Temperature-conditional nuclear mutation of Chlamydomonas reinhardtii decreases the CO2/O2 specificity of chloroplast ribulose-bisphosphate carboxylase/oxygenase. Planta 193:313-319

CC-4643 68-11AR mt- (rbc-2-68-11AR) non-pf2 progeny used for genetic analysis

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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