* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-599 nic1 mt+
$30.00
From Kathleen Peterson and W.T. Ebersold, UCLA, June 1978
Phenotype: requires nicotinamide
The nic1 mutation was obtained by Eversole, and is one of the few genetic markers for linkage group XV. This strain has given relatively high lethality in some crosses, but is easy to score by sensitivity to 3-acetyl pyridine.
Lin et al. demonstrated that this is a mutation in the gene encoding nicotinate-/nicotinamide-nucleotide adenylyltransferase.
Eversole RA (1956) Biochemical Mutants of Chlamydomonas reinhardi. Amer J Bot 43:404-407
Lin H, Kwan AL, Dutcher SK (2010) Synthesizing and salvaging NAD: lessons learned from Chlamydomonas reinhardtii. PLoS Genet 6