CC-4647 70-5Y mt+ (rbc-4-70-5Y) original isolate

$30.00

From Robert J. Spreitzer, University of Nebraska, March 2014

The 70-5Y nuclear mutant was isolated by Spreitzer et al. (1992) by screening a collection of acetate-requiring mutants for lack of complementation with a known Rubisco rbcL mutant (e. g., Spreitzer and Ogren 1983; Spreitzer et al. 1988). 70-5Y grows on minimal medium at 25 °C, but dies on minimal medium at 35 °C. When grown with acetate medium at 35 °C, it has normal whole-chain electron transport and chlorophyll levels, but lacks Rubisco holoenzyme. Purified Rubisco has altered catalytic properties. The 70-5Y mutation is not in the Rubisco rbcS genes, and it is not linked to the 68-11AR and 72-7JJ Rubisco nuclear-mutant genes (Hong 1996). This strain has been maintained with acetate medium in darkness since its isolation.

Hong S (1996) Nuclear mutations affect the catalysis, stability, and expression of chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Ph. D. thesis, University of Nebraska

Spreitzer RJ, Al-Abed SR, Huether MJ (1988) Temperature-sensitive, photosynthesis-deficient mutants of Chlamydomonas reinhardtii. Plant Physiol 86:773-777

Spreitzer RJ, Ogren WL (1983) Rapid recovery of chloroplast mutations affecting ribulosebisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 80:6293-6297

Spreitzer RJ, Thow G, Zhu G, Chen Z, Gotor C, Zhang D, Hong S (1992) Chloroplast and nuclear mutations that affect Rubisco structure and function in Chlamydomonas reinhardtii. Murata N ed Research in Photosynthesis 3:593-600