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CC-4648 70-5Y mt+ (rbc-4-70-5Y) non-pf2 progeny used for genetic analysis

Name: CC-4648 70-5Y mt+ (rbc-4-70-5Y) non-pf2 progeny used for genetic analysis
SKU: CC-4648
Availability: InStock

$30.00

From Robert J. Spreitzer, University of Nebraska, March 2014

Phenotype: requires acetate at 35 °C, temperature-conditional

The 70-5Y nuclear mutant was isolated by Spreitzer et al. (1992) by screening a collection of acetate-requiring mutants for lack of complementation with a known Rubisco rbcL mutant. 70-5Y grows on minimal medium at 25 °C, but dies on minimal medium at 35 °C. The mutant was crossed with pf2 mt- to confirm mendelian inheritance, and this mt+ progeny clone was retained for further analysis (Hong 1996). This strain has been maintained with acetate medium in darkness since its isolation.

Hong S (1996) Nuclear mutations affect the catalysis, stability, and expression of chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Ph. D. thesis, University of Nebraska

Spreitzer RJ, Thow G, Zhu G, Chen Z, Gotor C, Zhang D, Hong S (1992) Chloroplast and nuclear mutations that affect Rubisco structure and function in Chlamydomonas reinhardtii. Murata N ed Research in Photosynthesis 3:593-600

CC-4648 70-5Y mt+ (rbc-4-70-5Y) non-pf2 progeny used for genetic analysis

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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