CC-4654 prk1-R64C pf2 mt+ (12-2B) pf2 progeny used for genetic analysis
$30.00
From Robert J. Spreitzer, University of Nebraska, March 2014
Phenotype: requires acetate, sensitive to light
This strain was recovered from a cross between the original 12-2B mt+ isolate (CC-1227) and pf2 mt-. It has been maintained on acetate medium in darkness since its isolation. The 12-2B mutant was recovered as a light-sensitive, acetate-requiring mutant (Spreitzer and Mets 1981), and was subsequently found to lack phosphoribulokinase activity (Salvucci and Ogren 1985) due to a missense mutation (R64C; CGC-TGC) in the prk1 gene (Roesler et al. 1992).
Roesler K, Marcotte B, Ogren W (1992) Functional importance of arginine 64 in Chlamydomonas reinhardtii phosphoribulokinase. Plant Physiol 98:1285-1289
Salvucci M, Ogren W (1985) A Chlamydomonas reinhardtii mutant with catalytically and structurally altered ribulose-5-phosphate kinase. Planta 165:340-347
Spreitzer RJ, Mets L (1981) Photosynthesis-deficient mutants of Chlamydomonas reinhardtii with associated light-sensitive phenotypes. Plant Physiol 67:565-569