CC-4656 prk1-Δ50 mt+ (69-2P, original isolate)
$30.00
From Robert J. Spreitzer, University of Nebraska, March 2014
Phenotype: requires acetate, sensitive to light
A collection of over 350 acetate-requiring mutants (Spreitzer et al. 1988) was screened for complementation (Spreitzer and Ogren 1983) with the prk1-R64C mt- strain (12-2B mt-) (Smith 2000). Mutant 69-2P failed to complement, and lacked phosphoribulokinase activity and protein. Bryan D. Smith in Spreitzer’s group sequenced prk1, and discovered that 69-2P results from a 50-bp deletion (nucleotides 1296 through 1345 relative to the first nucleotide encoding the start codon; GenBank: AF228914.1) (Smith 2000). This strain has been maintained with acetate medium in darkness since its isolation.
Spreitzer RJ, Al-Abed SR, Huether MJ (1988) Temperature-sensitive, photosynthesis-deficient mutants of Chlamydomonas reinhardtii. Plant Physiol 86:773-777
Spreitzer RJ, Ogren WL (1983) Rapid recovery of chloroplast mutations affecting ribulosebisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 80:6293-6297
Smith BD (2000) Isolation and analysis of Chlamydomonas reinhardtii phosphoribulokinase mutants and revertants. Undergraduate Honors Thesis, Department of Biochemistry, University of Nebraska (available from the Chlamydomonas Resource Center)