CC-4659 prk1-Δ50 cwd mt- (69-2P)
$30.00
From Robert J. Spreitzer, University of Nebraska, March 2014
Phenotype: requires acetate, sensitive to light, lacks the cell wall
Bryan D. Smith in Spreitzer’s group transformed prk1-Δ50 cwd arg7-8 mt- with pARG7.8 (Gumpel et al. 1994) to eliminate the Arg requirement. This prk1-Δ50 cwd mt- strain lacks phosphoribulokinase activity and protein due to a 50-bp deletion in prk1 (Smith 2000), and may serve as a host for transformation with engineered prk1 genes. It has been maintained with acetate medium in darkness since its isolation.
Gumpel NJ, Rochaix JD, Purton S (1994) Studies on homologous recombination in the green alga Chlamydomonas reinhardtii. Curr Genet 26:438-442
Smith BD (2000) Isolation and analysis of Chlamydomonas reinhardtii phosphoribulokinase mutants and revertants. Undergraduate Honors Thesis, Department of Biochemistry, University of Nebraska (available from the Chlamydomonas Resource Center)