CC-4660 prk1-E267K mt- (R190-1C revertant of F-60)
$30.00
From Robert J. Spreitzer, University of Nebraska, March 2014
Following MMS mutagenesis of prk1-E267Amber mt- (GAG-TAG), Bryan Smith in Spreitzer’s group selected photosynthesis-competent revertants at a frequency of 1 X 10E-7 cells (Smith 2000). Revertant R190-1C has reduced photosynthetic growth relative to wild type, but has a normal level of phosphoribulokinase protein. It was found to result from a pseudoreversion substitution that converts the amber mutation (TAG) to Lys (AAG). The mutant protein has not been analyzed. This strain has been maintained with acetate medium in darkness since its isolation.
Smith BD (2000) Isolation and analysis of Chlamydomonas reinhardtii phosphoribulokinase mutants and revertants. Undergraduate Honors Thesis, Department of Biochemistry, University of Nebraska (available from the Chlamydomonas Resource Center)