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CC-4662 prk1-I28M/R64C mt+ (R175-3A revertant of 12-2B)

Name: CC-4662 prk1-I28M/R64C mt+ (R175-3A revertant of 12-2B)
SKU: CC-4662
Availability: InStock

$30.00

From Robert J. Spreitzer, University of Nebraska, March 2014

Following MMS mutagenesis of prk1-R64C mt+ (CGC-TGC), Bryan Smith in Spreitzer’s group selected photosynthesis-competent revertants at a frequency of 1 X 10E-8 cells (Smith 2000). Revertant R175-3A has reduced photosynthetic growth relative to wild type. It was found to result from intragenic suppression that converts phosphoribulokinase Ile-28 (ATC) to Met (ATG). The mutant protein has not been analyzed. This strain has been maintained with acetate medium in darkness since its isolation.

Smith BD (2000) Isolation and analysis of Chlamydomonas reinhardtii phosphoribulokinase mutants and revertants. Undergraduate Honors Thesis, Department of Biochemistry, University of Nebraska (available from the Chlamydomonas Resource Center)

Locus:
AC214 [PRK1]
Chromosome:
12

CC-4662 prk1-I28M/R64C mt+ (R175-3A revertant of 12-2B)

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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