CC-4665 prk1-R64C/S148F mt+ (R212-14A revertant of 12-2B)
$30.00
From Robert J. Spreitzer, University of Nebraska, March 2014
Following MMS mutagenesis of prk1-R64C mt+ (CGC-TGC), Bryan Smith in Spreitzer’s group selected photosynthesis-competent revertants at a frequency of 1 X 10E-8 cells (Smith 2000). Revertant R212-14A has reduced photosynthetic growth relative to wild type. It was found to result from intragenic suppression that converts phosphoribulokinase Ser-148 (TCC) to Phe (TTC). The mutant protein has not been analyzed. This strain has been maintained with acetate medium in darkness since its isolation.
Smith BD (2000) Isolation and analysis of Chlamydomonas reinhardtii phosphoribulokinase mutants and revertants. Undergraduate Honors Thesis, Department of Biochemistry, University of Nebraska (available from the Chlamydomonas Resource Center)