* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-4666 rca1Δ mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, March 2014
Phenotype: requires elevated CO2 for photosynthetic growth, zeocin resistant
This Rubisco activase knock-out mutant was received from Jim Moroney in 2006 (Pollock et al. 2003). It has been maintained with acetate medium in darkness to prevent selection for extragenic suppressors. The CO2-requiring phenotype may be easier to observe with 10-25 µM ethoxzolamide in the minimal medium plates.
Pollock SV, Colombo SL, Prout DL, Godfrey AC, Moroney JV (2003) Rubisco activase is required for optimal photosynthesis in the green alga Chlamydomonas reinhardtii in a low-CO2 atmosphere. Plant Physiol 133:1854-1861