* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-4670 rbcS1-HB cwd mt-
$30.00
From Robert J. Spreitzer, University of Nebraska, March 2014
Phenotype: altered CO2 assimilation
This mutant has the Rubisco small subunit replaced with an engineered spinach small subunit that has alpha-helix B replaced with the Chlamydomonas alpha-helix B (Meyer et al. 2012). It lacks the pyrenoid and the CO2 concentrating mechanism (CCM), and requires elevated CO2 for a wild-type level of photosynthetic growth. The strain has been maintained with acetate medium in darkness since its creation.
Meyer MT, Genkov T, Skepper JN, Jouhet J, Mitchell MC, Spreitzer RJ, Griffiths H (2012) Rubisco small-subunit α-helices control pyrenoid formation in Chlamydomonas. Proc Natl Acad Sci USA 109:19474-19479