CC-4673 12-1C mt- (ca1, CAH, cia3, cia4) non-pf2 progeny used for genetic analysis
$30.00
From Robert J. Spreitzer, University of Nebraska, March 2014
Phenotype: requires elevated CO2 for photosynthetic growth
Mutant 12-1C mt+ was crossed with pf2 mt-, and this mt- progeny clone was retained for further genetic analysis. Mutant 12-1C was originally recovered as an acetate-requiring mutant (Spreitzer and Mets 1981). Subsequent screening found it to be rescued by elevated CO2, and deficient in carbonic anhydrase activity (Spalding et al. 1983). This strain has been maintained with acetate medium in darkness since its isolation.
Spreitzer RJ, Mets L (1981) Photosynthesis-deficient mutants of Chlamydomonas reinhardtii with associated light-sensitive phenotypes. Plant Physiol 67:565-569
Spalding MH, Spreitzer RJ, Ogren WL (1983) Carbonic anhydrase-deficient mutant of Chlamydomonas reinhardii requires elevated carbon dioxide concentration for photoautotrophic growth. Plant Physiol 73:268-272