* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-4682 65-6A mt-
$30.00
From Robert J. Spreitzer, University of Nebraska, March 2014
Phenotype: requires elevated CO2 for photosynthetic growth at 35 °C
This is an mt- progeny clone of a cross between 65-6A mt+ and “wild-type” mt-. It grows on minimal medium at 25 °C, but dies on minimal medium at 35 °C. It can be rescued on minimal medium at 35 °C with 5% CO2 in air. It may be defective in the CO2-concentrating mechanism (CCM), but has not been analyzed. This nuclear mutant has been maintained with acetate medium in darkness since its isolation.
Spreitzer RJ, Al-Abed SR, Huether MJ (1988) Temperature-sensitive, photosynthesis-deficient mutants of Chlamydomonas reinhardtii. Plant Physiol 86:773-777