CC-4701 rbcL∆-25B1 pf2 mt- (pf2 progeny used for genetic analysis)
$30.00
From Robert J. Spreitzer, University of Nebraska, June 2014
Phenotype: requires acetate, sensitive to light
This 25B1 pf2 mt- progeny clone, which is green in the dark, was recovered from a cross between 25B1 mt+ (CC-2803) and pf2 mt- (Zhu and Spreitzer 1994). The 25B1 Rubisco rbcL knock-out mutant was created by chloroplast transformation and insertional mutagenesis (Newman et al. 1991). This strain has been maintained with acetate medium in darkness since its isolation.
Newman SM, Gillham NW, Harris EH, Johnson AM, Boynton JE (1991) Targeted disruption of chloroplast genes in Chlamydomonas reinhardtii. Molec Gen Genet 230:65-74
Zhu G, Spreitzer RJ (1994) Directed mutagenesis of chloroplast ribulose-bisphosphate carboxylase/oxygenase: Substitutions at large subunit asparagine 123 and serine 379 decrease CO2/O2 specificity. J Biol Chem 269:3952-3956