CC-4713 rbcL-C449S/C459S mt+ (Moreno)
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Phenotype: requires acetate
Directed mutagenesis and chloroplast transformation of rbcL-W66Amber mt+ (Spreitzer et al. 1985) were used to create C449S (TGT-AGC) and C459S (TGT-TCT) substitutions in the Rubisco large subunit to investigate stress-induced degradation of Rubisco (Marin-Navarro and Moreno 2006). This strain was received from Joaquin Moreno in 2011. Although this mutant was originally recovered on minimal medium and could grow photosynthetically (Marin-Navarro and Moreno 2006), a check of its phenotype in Spreitzer’s laboratory revealed that it now requires acetate for growth. Some photosynthesis-competent Rubisco mutants have been previously observed to become acetate-requiring after many years of culture in darkness.
Marin-Navarro J, Moreno J (2006) Cysteines 449 and 459 modulate the reduction–oxidation conformational changes of ribulose 1·5-bisphosphate carboxylase/oxygenase and the translocation of the enzyme to membranes during stress. Plant Cell Environ 29:898-908