* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-4714 rbcL-C449S/C459S mt+ (Spreitzer)
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Girish Rasineni in Spreitzer’s group recovered this mutant by transforming rbcL∆-MX3312 mt+ (CC-4696) with plasmid pLS-C449S/C459S (Marin-Navarro and Moreno 2006) obtained from Joaquin Moreno in 2011. This Rubisco large-subunit mutant can grow photosynthetically on minimal medium like the original rbcL-C449S/C459S mutant previously described by Marin-Navarro and Moreno (2006).
Marin-Navarro J, Moreno J (2006) Cysteines 449 and 459 modulate the reduction–oxidation conformational changes of ribulose 1·5-bisphosphate carboxylase/oxygenase and the translocation of the enzyme to membranes during stress. Plant Cell Environ 29:898-908