CC-4718 rbcS1-C83S mt-
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Using plasmid pSS1, which contains rbcS1 that encodes Rubisco small-subunit 1, and rbcS∆-T60-3 mt- (CC-4415) as the host strain (Khrebtukova and Spreitzer 1996), directed mutagenesis and nuclear-gene transformation were used to create a C83S substitution in the Rubisco small subunit (Moreno and Spreitzer, unpublished). The mutant may be useful for studying redox modulation of Rubisco (reviewed by Moreno et al. 2008). This strain has been maintained with acetate medium in darkness since being isolated in 2002.
Khrebtukova I, Spreitzer RJ (1996) Elimination of the Chlamydomonas gene family that encodes the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Proc Natl Acad Sci USA 93:13689-13693
Moreno J, García-Murria MJ, Marín-Navarro J (2008) Redox modulation of Rubisco conformation and activity through its cysteine residues. J Exp Bot 59:1605-1614