CC-4720 rbcL-C284S mt+
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Directed mutagenesis and chloroplast transformation of rbcL∆-25B1 mt+ (CC-4700) were used to create a C284S substitution in the Rubisco large subunit by standard procedures (Du and Spreitzer 2000), and photosynthesis-competent colonies were selected on minimal medium. After many rounds of cloning for homoplasmicity, this strain was retained for future study (Moreno and Spreitzer, unpublished). The mutant may be useful for studying redox modulation of Rubisco (reviewed by Moreno et al. 2008). It has been maintained with acetate medium in darkness since being isolated in 2002.
Du Y, Spreitzer RJ (2000) Suppressor mutations in the chloroplast-encoded large subunit improve the thermal stability of wild-type ribulose-1,5-bisphosphate carboxylase/ oxygenase. J Biol Chem 275:19844-19847
Moreno J, García-Murria MJ, Marín-Navarro J (2008) Redox modulation of Rubisco conformation and activity through its cysteine residues. J Exp Bot 59:1605-1614