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CC-4724 rbcS1-I58K mt-

Name: CC-4724 rbcS1-I58K mt-
SKU: CC-4724
Availability: InStock

$30.00

From Robert J. Spreitzer, University of Nebraska, July 2014

Using plasmid pSS1, which contains rbcS1 that encodes Rubisco small-subunit 1, and rbcS∆-T60-3 mt- (CC-4690) as the host strain (Khrebtukova and Spreitzer 1996), directed mutagenesis and nuclear-gene transformation were used to create an I58K substitution (ATC-AAG) in the Rubisco small subunit (Esquivel et al. 2013). This strain has been maintained with acetate medium in darkness since its isolation.

Esquivel MG, Genkov T, Nogueira AS, Salvucci ME, Spreitzer RJ (2013) Substitutions at the opening of the Rubisco central solvent channel affect holoenzyme stability and CO2/O2 specificity but not activation by Rubisco activase. Photosynth Res 118:209-218

Khrebtukova I, Spreitzer RJ (1996) Elimination of the Chlamydomonas gene family that encodes the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Proc Natl Acad Sci USA 93:13689-13693

Locus:
RBCS1
Chromosome:
2

CC-4724 rbcS1-I58K mt-

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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