CC-4726 rbcS1-I58W3 mt-
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Phenotype: requires acetate at 35 °C, temperature-conditional
Using plasmid pSS1, which contains rbcS1 that encodes Rubisco small-subunit 1, and rbcS∆-T60-3 mt- (CC-4690) as the host strain (Khrebtukova and Spreitzer 1996), directed mutagenesis and nuclear-gene transformation were used to replace Ile-58 with three Trp residues (ATC-TGGTGGTGG) in the Rubisco small subunit (Esquivel et al. 2013). This strain has been maintained with acetate medium in darkness since its isolation.
Esquivel MG, Genkov T, Nogueira AS, Salvucci ME, Spreitzer RJ (2013) Substitutions at the opening of the Rubisco central solvent channel affect holoenzyme stability and CO2/O2 specificity but not activation by Rubisco activase. Photosynth Res 118:209-218
Khrebtukova I, Spreitzer RJ (1996) Elimination of the Chlamydomonas gene family that encodes the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Proc Natl Acad Sci USA 93:13689-13693