CC-4728 rbcS1-R59A mt-
$30.00
Directed mutagenesis and nuclear-gene transformation of rbcS∆-T60-3 mt- (Khrebtukova and Spreitzer 1996) were used to create an R59A substitution (CGC-GCC) in the betaA-betaB loop of the Rubisco small subunit (Spreitzer et al. 2001; Spreitzer 2003). This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.
Khrebtukova I, Spreitzer RJ (1996) Elimination of the Chlamydomonas gene family that encodes the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Proc Natl Acad Sci USA 93:13689-13693
Spreitzer RJ (2003) Role of the small subunit in ribulose-1,5-bisphosphate carboxylase/oxygenase. Arch Biochem Biophys 414:141-149
Spreitzer RJ, Esquivel MG, Du YC, McLaughlin PD (2001) Alanine-scanning mutagenesis of the small-subunit betaA-betaB loop of chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase: Substitution at Arg-71 affects thermal stability and CO2/O2 specificity. Biochemistry 40:5615-5621