CC-4739 rbcS1-ABAN mt-
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Phenotype: requires acetate at 35 °C, temperature-conditional
Genetic engineering and nuclear-gene transformation of rbcS∆-T60-3 mt- (CC-4415) were used to replace the betaA-betaB loop of the Chlamydomonas Rubisco small subunit with the loop from Synechococcus Rubisco, which causes a decrease in Rubisco CO2/O2 specificity and holoenzyme stability (Karkehabadi et al. 2005a). The mutant can grow on minimal medium at 25 °C, but dies on minimal medium at 35 °C. This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.
Karkehabadi S, Peddi SR, Anwaruzzaman M, Taylor TC, Cederlund A, Genkov T, Andersson I, Spreitzer RJ (2005a) Chimeric small subunits influence catalysis without causing global conformational changes in the crystal structure of ribulose-1,5-bisphosphate carboxylase/oxygenase. Biochemistry 44:9851-9861