CC-4740 rbcS1-ABSO mt-
$30.00
From Robert J. Spreitzer, University of Nebraska, July 2014
Genetic engineering and nuclear-gene transformation of rbcS∆-T60-3 mt- (CC-4415) were used to replace the betaA-betaB loop of the Chlamydomonas Rubisco small subunit with the loop from spinach Rubisco, which causes a decrease in Rubisco carboxylation catalytic efficiency (Karkehabadi et al. 2005a; Spreitzer et al. 2005). This strain has been maintained with acetate medium in darkness to prevent selection for secondary mutations that may improve Rubisco function or stability.
Karkehabadi S, Peddi SR, Anwaruzzaman M, Taylor TC, Cederlund A, Genkov T, Andersson I, Spreitzer RJ (2005a) Chimeric small subunits influence catalysis without causing global conformational changes in the crystal structure of ribulose-1,5-bisphosphate carboxylase/oxygenase. Biochemistry 44:9851-9861
Spreitzer RJ, Peddi SR, Satagopan S (2005) Phylogenetic engineering at an interface between large and small subunits imparts land-plant kinetic properties to algal Rubisco. Proc Natl Acad Sci USA 102:17225-17230